Development of a PCR assay specific for Peptostreptococcus anaerobius
نویسندگان
چکیده
منابع مشابه
Extractable antigen shared by Peptostreptococcus anaerobius strains.
Extracts from several species of gram-positive cocci were prepared by a modification of the Rantz-Randall autoclave method and tested for reactions with rabbit anti-Peptostreptococcus anaerobius (ATCC 27337 and VPI 5737) sera in a capillary precipitin test. Antigen preparations from two reference strains of P. anaerobius (ATCC 27337 and VPI 5737) and six clinical isolates of P. anaerobius react...
متن کاملCellular fatty acids of Peptococcus variabilis and Peptostreptococcus anaerobius.
Cellular fatty acids of Peptococcus variables and Peptostreptococcus anaerobius were identified by gas chromatography, mass spectrometry, and associated analytical techniques. Iso- and anteiso-branched-chain acids were major components in both species.
متن کاملProduction of p-hydroxyhydrocinnamic acid from tyrosine by Peptostreptococcus anaerobius.
Peptostreptococcus anaerobius was found to metabolize tyrosine to p-hydroxyhydrocinnamic acid [3-(p-hydroxyphenyl)propionic acid]. This acid was detected in spent growth media by gas-liquid chromatography, and its identity was confirmed by mass spectrometry.
متن کاملDevelopment of SYBR Green I Based Real-Time RT-PCR Assay for Specific Detection of Watermelon silver mottle Virus
Background: Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. Objectives: Development of a highly sensitive and reliable detection method for WSMoV. Materials and Methods: Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...
متن کاملDevelopment of a Sensitive Quantitative Competitive PCR Assay for Detection of Human Cytomegalovirus DNA
Accurate and rapid diagnosis of human cytomegalovirus (HCMV) disease in immunocompromised patients has remained as a challenge. Quantitative competitive PCR (QC-PCR) methods for detection of HCMV in these individuals have improved the positive and negative predictive values of PCR for diagnosis of HCMV disease. In this study we used QC-PCR assay, using a co-amplified DNA standard, to quantitate...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 2002
ISSN: 0022-2615,1473-5644
DOI: 10.1099/0022-1317-51-12-1097